Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the Cell

<p>Neurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body.