Conservation of Formation of Nuclear Actin Filaments

<div><p>(A) PK15s infected with PRV expressing GFP-VP26, fixed at 9 hpi. Asterisks show cells that are infected and show short actin filaments that appear to associate with nuclear membrane. Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.1 μm apart. Scale bar = 20 μm.</p><p>(B) Enlarged image of nucleus labeled with yellow asterisk in (A) Arrowhead indicates nuclear actin filaments. Scale bar = 10 μm.</p><p>(C) SCG neurons infected with HSV-1 (KOS), fixed at 15 hpi. Cells were stained for the presence of viral capsid with anti-VP5 (capsid protein) antibody. Arrowheads indicate nuclear actin filaments. Each image is a 2-D projection from four consecutive layers in an image stack, taken 0.5 μm apart. Scale bar = 20 μm.</p>