Actin cytoskeleton is rearranged in the center of MDV infection plaques but not in periphery.

<p>Immunofluorescence microscopy of non infected cells (column A) and BAC20-EGFP infected cells, after 4 days of growth on the CESCs monolayer are shown, in the center of a representative infection plaque (column B), or in the periphery (columns C–D). F-actin was stained with AlexaFluor594-Phalloidin (red) (line 2) and DNA with Hoechst 33342 (blue) (B1–D1). Infected cells express the EGFPVP22 protein (green) (line 3). Asterisks show infected cells still possessing stress fibers. Arrowheads show infected cells which present connections between their stress fibers and those of neighboring infected or uninfected cells. Note that pictures in columns B and C are a superposition of 6 z stacks of 0.250 µm each, whereas pictures A and D correspond to a unique cut. All scale bars, 20 µm.