Simulation with Lipase Double Mutants

<p>Both the alanine at 217 and the phenylalanine at 214 were mutated to serines, and the simulation was carried out in an aqueous medium. The lids do not close in an aqueous environment. Thus, by mutating the key contact residues to serines, the hydrophobicity of lid2 is lost, and it is unable to function as a trigger for the lids to converge and close.</p> <p>Red, lid1 RMSD; blue, lid2 RMSD; black, lid1-lid2 distance.