Pseudomonas aeruginosa lipase is a 29-kDa protein that, following the determination of its crystal structure, was postulated to have a lid that stretched between residues 125 and 148. In this paper, using molecular dynamics simulations, we propose that there exists, in addition to the above-mentioned lid, a novel second lid in this lipase. We further show that the second lid, covering residues 210-222, acts as a triggering lid for the movement of the first. We also investigate the role of hydrophobicity in the movement of the lids and show that two residues, Phe214 and Ala217, play important roles in lid movement. To our knowledge, this is the first time that a double-lid movement of the type described in our manuscript has been presented t...
Folding and cellular localization of many proteins of Gram-negative bacteria rely on a network of ch...
<p>Both the alanine at 217 and the phenylalanine at 214 were mutated to serines, and the simulation ...
International audienceLarge-scale conformational rearrangement of a lid subdomain is a key event in ...
<div><p><i>Pseudomonas aeruginosa</i> lipase is a 29-kDa protein that, following the determination o...
<p>(A) This figure represents the simulation results. The structure on the left (i) is the open form...
International audienceThe interfacial activation of many lipases at water/lipid interface is mediate...
Interfacial activation of Rhizomucor miehei lipase is accompanied by a hinge-type motion of a single...
Lipases naturally function at the interface formed between amphiphilic molecules and the aqueous env...
Lipases are enzymes that play fundamental roles in fat digestion and metabolism, and function at the...
In nonaqueous organic solvents, lipases can catalyze esterification reactions, which increase their ...
AbstractWe report the 1.7 Å resolution crystal structure of the Lip2 lipase from Yarrowia lipolytica...
<p>(A) This figure corresponds to the simulation of mutant Ala217Ser. The movement of the lids very ...
Lipases are essential and widely used biocatalysts. Hence, the production of lipases requires a deta...
Pancreatic lipase is a soluble globular protein that must undergo structural modifications be...
AbstractRhizomuor miehei Lipase (RmL) is a type of lipase that has a single lid segment playing impo...
Folding and cellular localization of many proteins of Gram-negative bacteria rely on a network of ch...
<p>Both the alanine at 217 and the phenylalanine at 214 were mutated to serines, and the simulation ...
International audienceLarge-scale conformational rearrangement of a lid subdomain is a key event in ...
<div><p><i>Pseudomonas aeruginosa</i> lipase is a 29-kDa protein that, following the determination o...
<p>(A) This figure represents the simulation results. The structure on the left (i) is the open form...
International audienceThe interfacial activation of many lipases at water/lipid interface is mediate...
Interfacial activation of Rhizomucor miehei lipase is accompanied by a hinge-type motion of a single...
Lipases naturally function at the interface formed between amphiphilic molecules and the aqueous env...
Lipases are enzymes that play fundamental roles in fat digestion and metabolism, and function at the...
In nonaqueous organic solvents, lipases can catalyze esterification reactions, which increase their ...
AbstractWe report the 1.7 Å resolution crystal structure of the Lip2 lipase from Yarrowia lipolytica...
<p>(A) This figure corresponds to the simulation of mutant Ala217Ser. The movement of the lids very ...
Lipases are essential and widely used biocatalysts. Hence, the production of lipases requires a deta...
Pancreatic lipase is a soluble globular protein that must undergo structural modifications be...
AbstractRhizomuor miehei Lipase (RmL) is a type of lipase that has a single lid segment playing impo...
Folding and cellular localization of many proteins of Gram-negative bacteria rely on a network of ch...
<p>Both the alanine at 217 and the phenylalanine at 214 were mutated to serines, and the simulation ...
International audienceLarge-scale conformational rearrangement of a lid subdomain is a key event in ...