IFNβ-Induced Changes in Gene Expression over Time

<div><p>(A) An unsupervised hierarchical clustering representation of the weighted difference in gene expression at each time point versus baseline. For each gene, the obtained differences were log normalized and multiplied by the <i>F</i>-statistic from an ANOVA (time effect) run previously (shown in [B]). The “heat” colored bar represents the absolute value of this difference. With the exception of <i>IFNAR1</i> (arrow), all genes showing a significantly different expression in at least one time point with respect to baseline were arranged in the same cluster (framed in blue).</p> <p>(B) List of all genes showing a significant time effect along with their <i>F</i>-statistic and <i>p</i>-values. Genes that were part of any triplet showing more than 80% prediction accuracy at <i>T</i> = 0 are in bold.</p> <p>(C) A continuous representation of the longitudinal average expression of two representative genes over all samples. <i>MX1</i> (̂) shows a marked departure from <i>T</i> = 0 and remains elevated for the rest of the observed period. This correlates well with the shading (#) displayed in the clustering shown in (A). In contrast, <i>IRF6</i> (•) displays an almost flat curve, consistent with its color in the clustering (*).</p>