TF promoter deletion analysis.

<p>(A) Schematic structure of TF promoter fused with Luciferase expression gene. hGAG effect on luciferase activity of B16F10 cells transfected with pTF-Luc (B), pTF/5′-d-Luc (C), pTF/A-d-Luc (D), pTF/AN-d-Luc (E) pTF/ANS-d-Luc (F). B16F10 cells were transfected with the constructed luciferase expression vector and treated with hGAG at the indicated concentration for 24 h. Luciferase activity was assayed. (G) Mutation of NF-κB binding site. Mutation of NF-κB binding site was performed using MutanBEST Kit (Takara) and the mutated pTF/NF-m-Luc was transfected into B16F10 cells. hGAG treatment and luciferase assay were carried out following the same procedure as above. Data are represented as mean ± S.E. from 3–5 independent experiments. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.