Promoter activity analyses of the GDP-fucose transporter gene.

<p><b>A.</b> Schematic representation of the luciferase reporter constructs carrying serial deletions of the upstream sequences of the GDP-fucose transporter gene in pGL-3 basic vector. The numbers indicate the upstream sequences relative to the transcription initiation site. Luciferase start codon is indicated. <b>B.</b> Luciferase activity assays of the constructs described in A. The constructs containing various upstream promoter sequences as in A were transfected into HEK293 cells. After 24 h, the cells were harvested for whole cell lysates, which were used for luciferase activity analyses with Luciferase Assay Systems. The relative luciferase activity was measured by microplate luminometer. Error bars represent standard deviation from four independent experiments. **, p<0.01 (t-test).