Recruitment of the tight junction protein ZO-1 at cell membrane after APC and thrombin exposure.

<p>Levels of ZO-1 protein in the membrane/cytoskeleton and cytosolic fractions were evaluated by western blot. A549 cells were pretreated with APC at 50 µg/ml or vehicle for 3 h, followed by thrombin (50 nM) or culture medium treatment for 5 min. Tubulin was used as an invariant control. Data from seven independent experiments are reported as mean values ± SEM. The results are expressed as a percentage of control (vehicle + culture medium group). The statistical significance of the results was assessed by one-way ANOVA (p = 0.038). * and # indicate significant differences between the vehicle + thrombin and APC + thrombin groups and between the vehicle + thrombin and control groups, respectively (p<0.05).