Multiple sequence alignment for <i>O. iheyensis</i> nicotinamidase (OiNIC) and related nicotinamidases.

<p>ESPript outputs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056727#pone.0056727-Gouet1" target="_blank">[48]</a> obtained with the crystallized nicotinamidase sequences retrieved from Uniprot database and later aligned with CLUSTAL-W <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056727#pone.0056727-Thompson1" target="_blank">[47]</a>. Sequences are grouped according to similarity. PDB codes were 3O90 for <i>S. pneumoniae</i> nicotinamidase (SpNIC), 1ILW for <i>P. horikoshii</i> nicotinamidase (PhPncA), 3PL1 for <i>M. tuberculosis</i> nicotinamidase (MtPncA), 2WT9 for <i>A. baumanii</i> nicotinamidase (AbPncA), 2H0R for <i>S. cerevisiae</i> nicotinamidase and 2R3J for <i>Leishmania infantum</i> nicotinamidase. Residues strictly conserved across nicotinamidase enzymes have a dark background. Symbols above blocks of sequences represent the secondary structure, springs represent helices and arrows represent β-strands. The residues forming the active site are indicated by triangles. Residues involved in the coordination of the metal ion are indicated by stars and residues forming the <i>cis</i>-peptide bond are indicated by diamonds.