Multiple sequence alignment of <i>M</i>. <i>tuberculosis</i> CysC.

<p>The sequence of mycobacterial CysC (top sequence) is aligned with the sequences of human bifunctional PAPS synthase 1 (PAPSS1, PDB ID 1xnj), the kinase domain of human PAPS synthase 2 (PAPSS2, 2ax4) and APS kinases from <i>A</i>. <i>thaliana</i> (4fxp), <i>P</i>. <i>chrysogenum</i> (1dj6), <i>T</i>. <i>denitrificans</i> (3cr8), <i>A</i>. <i>aeolicus</i> (2gks), and <i>A</i>. <i>pernix</i> (2yvu). Note that the alignment is shown for the APS kinase domains only in case of bifunctional enzymes. First residues included in the alignment are numbered for each sequence. White font with red background highlights identical residues and the black font with yellow background indicates similar residues. Residues Cys514, Cys549 and Cys556 of <i>M</i>. <i>tuberculosis</i> are highlighted by red boxes and marked with red triangles. Secondary structure elements for CysC from <i>M</i>. <i>tuberculosis</i> are shown on top. The figure was made using ESPript [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121494#pone.0121494.ref040" target="_blank">40</a>].