Oct4GFP and NanogGFP are expressed in mouse testes.

<p>(A) Cells from testes of GFP−/− control transgenic mice, Oct4GFP+/−, Oct4GFP+/+ and NanogGFP+ animals were isolated and analysed for GFP positivity by FACS. Representative flow cytometry analyses in which sideward scatter is plotted against GFP fluorescence are shown for each genotype. The percentage of GFP+ cells compared to GFP negative control testes is indicated in the gate. (B) GFP mRNA expression in control (n = 3), Oct4GFP+/− (n = 6), Oct4GFP+/+ (n = 2) and NanogGFP+ (n = 5) testes was analysed by qPCR. Circles represent individual samples, the bar indicates the mean value of all samples. (C) GFP+ cells can be detected in Oct4GFP+ and NanogGFP+ testes but not in control testis. Representative pictures of immunofluorescence staining with anti-GFP antibody are shown. Magnification 200×. The framed region is enlarged in the insert.