Expression of <i>Oct4-GFP</i> and other stem cell markers in skin-derived cells from <i>Oct4-GFP</i> transgenic mice.

<p>A. Detection of <i>GFP</i> and <i>Oct4</i> transcripts from newborn skin tissue. 1, ovary of non-transgenic mice (control); 2–4, skin of <i>Oct4-GFP</i> transgenic mice; N, negative control (PCR without reverse transcription for <i>Rp2</i>). B. GFP-positive cells located in a hair follicle of a new born mouse. C. H &E staining of the section in B showing the skin structure. D. Negative control in which the primary antibody was omitted. E. RT-PCR showing expression of a panel of genes in uncultured skin cells: 1, <i>Rp2</i>; 2, <i>Oct4</i>; 3, <i>Dazl</i>; 4, <i>Nanog</i>; 5, <i>Kit</i>; 6, <i>Stra8</i>; 7, <i>Scp3</i>. F,G. Representative flow cytometric analysis showing GFP-positive skin-derived stem cells at passage 0 (F) and 2 (G). Horizontal and vertical axes indicate fluorescence and number of events, respectively. H. RT-PCR detection of <i>Oct4</i> mRNA in the sorted GFP⁻ and GFP⁺ populations; <i>Rp2</i> was used as house keeping control. I. Detection of SSEA1 in skin-derived stem cells at passage 2 via flow cytometric analysis. Data are mean ± SE of 3 experiments. J. GFP positive stem cells (collected by cytometric flow sorting) depicting the morphology of germ cells including high nuclear to cytoplasm ratio. Scale bars: B–D and H, 50 µm.