Combinatorial histone modifications.

<p>(A) <i>PCC-based correlation of the dynamic histone occupancy profiles</i>. The bar charts show examples of pair wise correlation distributions between the dynamic profiles of the individual histone marks calculated analogously to the correlations with steady state mRNA levels (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003170#ppat-1003170-g003" target="_blank">Figure 3</a>). Line graphs give the randomized distribution of PCC. <i>P</i> value (P) and skewness (S) are shown below each graph. (B) <i>Hierarchical clustering of histone mark associations</i>. The heat maps represent hierarchical clustering of the skewness (S) values of the PCC distributions for every marked histone pair. High positive skew (red) and low negative skew (blue) indicate positive and negative correlations with the marked histone pairs, respectively. (C) <i>Sequential ChIP-on-chip for H3K9ac and H3K56ac</i>. The sequential ChIP-on-chip was carried out with ring stage parasites using anti-H3K56ac antibody followed by anti-H3K9ac antibody. Immunoprecipitation was also done using anti-H3K56ac antibody or anti-H3K9ac antibody separately. Data was averaged from 2 separate experiments and probes with ChIP/input signal ratio above 1 for bead control were subtracted for further analysis. The chart (above) shows the probes overlapping between the sequential ChIP and probes shared in common between both histone marks when used individually (ChIP/input >1). Chromosomal distribution (below) of H3K56ac (orange), H3K9ac (green) and sequential (purple) ChIP enrichment of probes as seen at chromosome 4. Each vertical line depicts the position of a probe on the chromosome.