MdLBD11 interacts with AtAS1 in a yeast two-hybrid assay.

<p>Yeast strains containing pGAD-AtAS1, pGBD-MdLBD11, and pGBD-AtAS2 were assayed for LacZ expression, pGAD-AtAS1 in combination with pGBD-AtAS2 was used as a positive control, and pGAD with pGBD-MdLBD11 was used as a negative control. Yeast grew on SD/−T/−L medium to select for both the bait and prey proteins (left). SD/−T/−L/−A/−H media allow the growth of only positively interacting clones. SD/−T/−L/−A/−H media plus X-gal allow the growth of only the positively interacting clones.