PKCε translocation is involved in isoflurane preconditioning.

<p>A PKCε translocation was associated with isoflurane-induced phosphorylation of ALDH2. (a) Representative of western blot analysis of phos-ALDH2, total ALDH2, β-actin, mitochondria PKCε (mito-PKCε) and total PKCε (from top lanes to bottom lanes). β-actin was used to demonstrate equal protein loading. (b) Quantification of the phos-ALDH2, normalized to the total ALDH2, and PKCε translocation to the mitochondria from 3 different experiments. B, C. Isoflurane-induced inhibition of LDH and CK-MB release by I/R was restored by PKCε v1–2. Serum LDH and CK-MB concentrations were analyzed. D. PKCε v1–2 inhibits the decrease in heart infarct size caused by isoflurane following I/R. Representative cross-sectional slices derived from a single heart. The infarct size normalized to the area at risk. Values are means ± S.E.M., n = 8 in each group. *P<0.05, **P<0.01 vs. the saline control group and ^#P<0.05, ^##P<0.01 with PKCε v1–2 vs. the corresponding group without PKCε v1–2.