Desflurane Preconditioning Induces Oscillation of NF-kB in Human Umbilical Vein Endothelial Cells

Background: Nuclear factor kappa B (NF-kB) has been implicated in anesthetic preconditioning (APC) induced protection against anoxia and reoxygenation (A/R) injury. The authors hypothesized that desflurane preconditioning would induce NF-kB oscillation and prevent endothelial cells apoptosis. Methods: A human umbilical vein endothelial cells (HUVECs) A/R injury model was used. A 30 minute desflurane treatment was initiated before anoxia. NF-kB inhibitor BAY11-7082 was administered in some experiments before desflurane preconditioning. Cells apoptosis was analyzed by flow cytometry using annexin V–fluorescein isothiocyanate staining and cell viability was evaluated by modified tertrozalium salt (MTT) assay. The cellular superoxide dismutases (SOD) activitiy were tested by water-soluble tetrazolium salt (WST-1) assay. NF-kB p65 subunit nuclear translocation was detected by immunofluorescence staining. Expression of inhibitor of NF-kB-a (IkBa), NF-kB p65 and cellular inhibitor of apoptosis 1 (c-IAP1), B-cell leukemia/lymphoma 2 (Bcl-2), cysteine containing aspartate specific protease 3 (caspases-3) and second mitochondrial-derived activator of caspase (SMAC/DIABLO) were determined by western blot. Results: Desflurane preconditioning caused phosphorylation and nuclear translocation of NF-kB before anoxia, on the contrary, induced the synthesis of IkBa and inhibition of NF-kB after reoxygenation. Desflurane preconditioning up-regulated the expression of c-IAP1 and Bcl-2, blocked the cleavage of caspase-3 and reduced SMAC release, and decrease