Carbachol depolarizes parasubicular neurons to threshold voltages.

<p><b>A.</b> Representative recording of membrane potential from a layer II parasubicular neuron (same cell as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058901#pone-0058901-g001" target="_blank">Figure 1A</a>₁) in response to a 2.5 min bath application of 25 µM CCh (white bar). Note that repeated hyperpolarizing current pulses were used to monitor input resistance during recordings, and that steady negative hyperpolarizing current was used to return the cell to baseline voltages to assess changes in input resistance. <b>B</b>. Group data show the mean depolarization of resting membrane potential in response to different concentrations of CCh in separate groups of neurons. The lowest concentration of 5 µM CCh failed to produce a significant depolarization, but higher doses reliably depolarized layer II PaS neurons (*: <i>p</i><0.05; **: <i>p</i><0.01).