Effects on cell growth after Au- or AgNPs of HNPCs.

<p><b>A.</b> Au- or AgNPs did not significantly affect the cell growth, presented as total numbers of live and dead cells, of HNPCs. The results are presented as mean values (n = 2−3 samples from three independent experiments). Error bars represent ± SD. White bars, live cells; grey bars, dead cells; Au 20∶50, gold 20 nm and 50 particles/cell; Au 20∶800, gold 20 nm and 800 particles/cell; Au 80∶50, gold 80 nm and 50 particles/cell; Au 80∶800, gold 80 nm and 800 particles/cell; Ag 20∶50, silver 20 nm and 50 particles/cell; Ag 20∶800, silver 20 nm and 800 particles/cell; Ag 80∶50, silver 80 nm and 50 particles/cell; Ag 80∶800, silver 80 nm and 800 particles/cell; AgNO₃ 0.5, silver nitrate 0.5 mg/ml; AgNO₃ 1.0, silver nitrate 1.0 mg/ml; AgNO₃ 5.0, silver nitrate 5.0 mg/ml. *<i>p<</i>0.05 compared to control. <b>B.</b> Both Au- and AgNPs affect the morphology of HNPCs when grown as neurospheres. AgNO₃ was toxic to HNPCs. NPs or AgNO₃ were added 48 h after seeding. The shown data are representative of three independent experiments. 50 p/c, 50 particles/cell; 800 p/c, 800 particles/cell; Scale bar equals 400 µm.