Analysis of stromal and epithelial markers in WT or <i>Lxrαβ−/−</i> MPECs.

<p>(A) WT and <i>Lxrαβ−/−</i> MPECs and WT MEFs were immunostained using anti-E-cadherin (E-Cad), anti-Cytokeratin8 (CK8) and anti-αTubulin (Tub) antibodies. Scale bar 100 µm. (B) mRNA relative levels of <i>Tenascin, Snail, Keratin 8</i> and <i>E-cadh</i> were measured in WT MEFs, WT (+/+) and <i>Lxrαβ−/−</i> MPECs by qPCR. *p<0.05, **p<0.01, ***p<0.001 in Student’s <i>t</i> test. Error bars represent mean ± SEM. (qPCR analysis results from 3 independant experiments and was normalized using <i>36b4</i> gene) (C) Western blot analysis was performed on WT MEFs, WT (+/+) and <i>Lxrαβ−/−</i> MPECs using CK8, Psca antibodies. βActin was used as a loading control.