Analysis of E-cadherin expression and β4 expression on K8 up−/down-regulation.

<p>E-cadherin expression: Representative confocal images of E-cadherin in (<b>A</b>) K8 over-expressed MDA MB 435 (K8C1) and vector control (K8Vc) clones. (<b>C</b>) MDA MB 468 K8 down-regulated (ShC1) and vector control (Vc) clones. (<b>E</b>) MCF10A K8 down-regulated (MC1) and vector control (MVc) clones. Scale bar: 10 µm. Note: Cytoplasmic localization of E-cadherin in K8 down-regulated MDA MB 468 clones, Nuclei (blue) is stained with DAPI. Western blot analysis of E-cadherin: (<b>B</b>) MDA MB 435 K8 over-expressed (K8C1, C2 and C3) and vector control (K8Vc) clones. MCF7 served as positive control. (<b>D</b>) MDA MB 468 K8 down-regulated (ShC1, C2 and C3) and vector control (Vc) clones. (<b>F</b>) MCF10A K8 down-regulated (MshC1, C2 and C3) and vector control (MVc) clones. β-actin was used as loading control. <b>Note:</b> No change in E-cadherin levels on K8 up−/down-regulation. (<b>G</b>) Western blot analysis of β4 integrin: in MDA MB 435 K8 over-expressed (K8C1, C2 and C3) and vector control (K8Vc) clones and MDA MB 468 K8 down-regulated (ShC1, C2 and C3) and vector control (Vc) clones. β-actin was taken as loading control. <b>Note:</b> No change in levels of β4 integrin.