Transcriptional potential of CBAp, HS2-βp, and B19p6 promoters in human hematopoietic cells.

<p>Approximately 1×10⁴ cells were either infected with DM scAAV6 vectors (K562 cells) or WT scAAV6 vectors (human CD34⁺ cells) expressing the EGFP gene under the control of the three different promoters at 5×10³ vgs/cell (K562 cells) or 2×10⁴ vgs/cell (human CD34⁺ cells), respectively. Transgene expression in K562 cells (Panels A and B) and human CD34⁺ cells (Panels C and D) was determined 72 hrs post-infection by fluorescence microscopy and quantitated by the flow cytometry as described above. The original image magnifications were 100× (Panel A) and 200× (Panel B). *DM-scB19p6-EGFP vs. DM-scAAV6-HS2-βp-EGFP vectors, <i>p</i><0.01.