Transduction efficiency of WT and tyrosine-mutant scAAV6 vectors in human hematopoietic cells.

<p>Approximately 5×10³ K562 cells were either mock-infected, or infected with 5×10³ vgs/cell of WT or various tyrosine-mutant scAAV6-CBAp-EGFP vectors, and transgene expression was determined 48 hrs post-infection using a Zeiss fluorescence microscope (Panel A), and Accuri C6 flow cytometer (Panel B) (Original magnification, x100) Approximately 1×10⁴ primary human CD34⁺ cells were either mock-infected, or infected with 2×10⁴ vgs/cell of WT or various tyrosine-mutant scAAV6-CBAp-EGFP vectors under identical conditions, and transgene expression was determined 72 hrs post-infection by fluorescence microscopy (Panel C) (Original magnification, x200), and quantified by fluorescence-activated cell sorting (FACS) using a BD FACS Aria Flow Cytometer followed by processing with software FCS Express 4 (Panel D). *Y705+731F-scAAV6 vs. WT-scAAV6 vectors, <i>p</i><0.01.