Pepsin cleavage of the surface-displayed LO-B_n fusion proteins.

<p>(A) SDS-PAGE analysis of the pepsin-digested mixtures. Lanes 1–4 represent total cell proteins, and lanes 5–8 represent the pepsin-digested mixtures from <i>E. coli</i> BL21 (DE3) harboring pLO-B₀, -B₁, -B₂, and -B₃, respectively. Lane 9 represents the synthetic Buf IIIb-L, and lane M represents molecular weight markers. The <i>closed triangle</i> indicates a band corresponding to Buf IIIb-L monomer. (B) HPLC-chromatograms of the synthetic Buf IIIb-L (a) and the purified recombinant Buf IIIb-L monomers from the pepsin-digested mixtures from <i>E. coli</i> BL21 (DE3) harboring pLO-B₀, -B₁, -B₂, and -B₃, respectively (b–d). The <i>arrow</i> indicates a peak corresponding to Buf IIIb-L monomer.