Overexpression Rab4A impairs trafficking of FVIIa from early endosomes to the recycling compartment.

<p>CHO-EPCR cells were transduced with adenoviruses encoding wild-type Rab4A. The transduced cells were incubated with AF488-FVIIa (50 nM) for 1 h at 4°C, and then transferred to 37°C to induce internalization of the surface bound ligand. The permeabilized cells were immunostained for EPCR and Rab4A, and immunofluorescence and fluorescence of AF488-FVIIa were analyzed by confocal microscopy. The two right two panels are the digitally enlarged images of the insets of 2^nd and 3^rd panels to clearly illustrate differences in the intracellular localization of FVIIa at different time intervals. The arrow indicates the accumulation of AF488-FVIIa in the REC.