Effect of overexpression of wild-type, constitutively active, or dominant negative variant of Rab4A on accumulation of FVIIa in the recycling endosomal compartment.

<p>CHO-EPCR cells transduced with control adenoviruses (brown) or recombinant adenoviruses encoding wild-type (blue), constitutively active (green), or dominant negative (red) Rab4A were incubated with AF488-FVIIa (50 nM) for 1 h at 4°C, and allowed to internalize for varying time periods at 37°C. FVIIa accumulated in the REC was quantified by measuring the pixel density of the fluorescence of AF488-FVIIa in this compartment. * denotes that the value significantly differs from the values obtained in cells expressing endogenous Rab4A (<i>P<0.01</i>).