In vitro staining of brain sliced from transgenic 2576 AD mice using scFv-IC16, 6E10-Aβ-antibody and DAPI.

<p>(A). Pretreated deep frozen horizontal brain cryosections (20 µm thickness) from 9 months old Tg2576 mice were fixed in 4% paraformaldehyde, treated with 70%formic acid and either incubated with scFv-IC16 in a concentration of 400 nM or 6E10 in a concentration of 33 nM. As scFv-IC16 is fused with a His tag, the respective sections were incubated with Penta-His Mouse antibody. Detection was performed using goat anti mouse-Alexa488 using a fluorescent microscope (Leica LMD6000. Excitation Range: UV; blue; green. Excitation Filter: Bp 420/30; Bp 495/15; Bp 570/20) with camera (Leica DFC 310 FX). Images were processed with LAS software (Leica Application Suite, V.4.0.0) and ImageJ (1.45 s). Scale bar: 50 µm. B: In vitro staining of brain sliced from transgenic 2576 AD mice using scFv-IC16 and a respective wildtype control mouse. Scale bar:100 µm. C: Co-staining of brain sliced from transgenic 2576 AD mice using scFv-IC16 and anti Aβ(1-42) 1-11-3. As secondary antibodies, a mix of goat anti mouse-Alexa488 and goat anti rabbit-Alexa-568 was used. Scale bar: 50 µm.