Neurogenesis changes are not involved in the olfactory deficit of α-syn mice and rasagiline-induced improvement.

<p><b>A.</b> Quantification of newborn cells in the granule cell layer of the OB. Total number of BrdU positive cells was assessed every sixth section by stereology (counting frame 100 µm×100 µm; counting grid: 300 µm×300 µm). No difference between control and α-syn mice as well as no effect of rasagiline treatment was observed. N = 4-6 for each group aged 12 months. Statistic: one-way ANOVA, p = 0.66, F(3,14) = 0.54. <b>B.</b> BrdU staining in the olfactory bulb of WT and α-syn mice. Scale bars: 100 µm. <b>C. </b>Quantification of newborn neurons in the granule cell layer of the OB. The proportion of BrdU positive cells, which are also NeuN positive, was assessed by confocal microscopy. No difference between control and α-syn mice as well as no effect of rasagiline treatment was observed. On average, we analyzed 100 BrdU-positive cells in each animal, N = 3 mice in each group aged 12 months. Statistic: one-way ANOVA, p = 0.61, F(3,8) = 0.65. <b>D.</b> NeuN (green) and BrdU (red) double staining in the OB. Examples of NeuN-positive/BrdU positive-cells observed in WT and α-syn mice. Scale bars: 55.5 µm.