Gene expression changes under array conditions define a time course for studying transcriptional changes in EMT.

<p>Gene expression changes in treated cells are shown normalized to levels in vehicle controls (dashed grey line). All differences had reached significance by the end of the six-day time period except for E-cadherin in TGF-β1 treated HMLE Twist ER. <b>A.</b> Changes in E-cadherin and mesenchymal marker expression in HMLE Twist ER cells treated with 4OHT. <b>B.</b> Changes in E-cadherin and mesenchymal marker expression in HMLE Twist ER cells treated with TGF-β1. <b>C.</b> Changes in E-cadherin and mesenchymal marker expression in MCF-7 cells treated with TGF-β1. <b>D.</b> Decreased E-cadherin protein localization at the cell membrane in HMLE Twist ER cells treated with TGF-β1. Scale bar, 100 μm. Error bars indicate standard error of the mean.