LC3 II shows no change following GCase inhibition in rat embryonic cortical cultures.

<p>(A) Cultured cortical neurons (E18) were exposed to increasing doses of CBE (100, 200 µΜ) for 7 and 10 days respectively. Untreated cells served as control (ctl). Cells were lysed, separated with SDS-PAGE and immunoblotted with the LC3 polyclonal antibody. ERK = loading control. (B) Graphs show quantification of LC3 II immunoreactivity (vs. ERK) via densitometric analysis at the 7 and 10 day time points respectively. Statistical analysis made via One Way Anova showed no difference in the LC3 II levels for the corresponding doses and time points of CBE in comparison to the control samples (n = 6 samples/2 different culture preparations with 3 independent biological samples per condition).