LC3-II was detected with a monoclonal anti-LC3-II-FITC antibody (green).

<p>The sections were taken from the infarct regions of the ipsilateral hippocampus 1, 3, 6, 12 and 24 h following I/R. I/R increased the LC3-II-positive cells and LC3-II protein levels in the ischemic hippocampus after I/R in rats. I/R was induced by two-vessel occlusion. Representative photomicrographs of LC3-II immunofluorescence. Immunofluorescence of LC3-II was performed at 0–24 h after I/R. Images (magnification 200x) were taken from the same part of the ischemic hippocampus. (B) The quantitative analysis of the number of LC3-II-positive cells. The number of LC3-II-positive cells in the ischemic hippocampus was significantly increased in the ischemic rats compared to the sham rats. The data are expressed as percentage of the sham-operated animals and as the mean±SD, <i>n</i> = 6. The statistical analysis was performed using a one-way ANOVA. *<i>p</i> < 0.05, **<i>p</i> < 0.01 vs. sham group.