Detection of GPR87 expression in HCC cell lines and human primary cells by qRT-PCR and Western blot analysis.

<p>(A) Relative mRNA expression levels of GPR87 and CD133 were determined by quantitative polymerase chain reaction in the CD133⁺ and CD133⁻ populations sorted from the SMMC-7721, HCC-LY5 and MHCC-97L cell lines. (B) Western blot of GPR87 in the HCC cell lines and primary human cells. (C) Western blot of GPR87 in the CD133⁺ and CD133⁻ populations sorted from the SMMC-7721, HCC-LY5, SNU475 and PLC/RFP/5 cells. (D) The protein expression levels of GPR87 and CD133 were determined by Western blot analysis in the SMMC-7721-lenti-GPR87 and HCC-LY5-lenti-GPR87 cells. (E) The relative mRNA expression of GPR87 and CD133 were determined by quantitative polymerase chain reaction in the SMMC-7721-lenti-GPR87 and HCC-LY5-lenti-GPR87 cells. (F) The table showed the correlation between CD133 and GPR87 expression in HCC tissues.