Detection of Tβ4 in the activated HSCs and human fibrotic liver.

<p>(A) QRT-PCR analysis of mRNA form human hepatic stellate cell line (LX-2) and human hepatocellular carcinoma cell line (HepG2). Mean±SD results are graphed. Data represent the mean±SD of three independent experiments (**p<0.005 vs. HepG2). (B) Western blot analysis of Tβ4 (GAPDH was used as an internal control). Data shown represent one of three experiments with similar results. (C) Representative immunostaining images for Tβ4 in LX-2 cells and HepG2 cells. Brown color indicated Tβ4-positive cells. (X40) (D) QRT-PCR analysis of mRNA from mouse primary HSCs (Day 0: freshly isolated HSCs / Day 7: cultured primary HSCs for 7 days. Mean±SD results are graphed. Data represent the mean±SD of three independent experiments (**p<0.005). (E) QRT-PCR analysis of RNA isolated from healthy livers (HL, n = 3) and fibrotic livers (stage 4 of fibrosis, n = 13). Mean±SD results are graphed and data represent the mean±SD of three independent experiments (**p<0.005).