AFM image of individual Golgi cisternae.

<p>(A) AFM Image of a globular membranous cisterna. Scale bar is 400 nm. (B) Magnification of the cisterna in (A). Scale bar is 200 nm. (C) Higher magnification of the blue boxed area in (B). Scale bar is 100 nm. (D, E, F) Cross section analysis along the green line drawn in (A) (B) and (C). (G) AFM image of a flat membranous cisterna. Blue arrows point to protein particles underneath the cisterna. Scale bar is 500 nm. (H) AFM image of an oval-shaped membranous cisterna with peripheral vesicles. Scale bar is 500 nm. (I and J) Cross section analysis along the green line drawn in images (G) and (H). Green arrows in Fig. 2 point to buds or interconnected vesicles around the cisterna.