Branched cisternae.

<p><b>A,B.</b> Tomographic slices of a portion of the Golgi ribbon from chemically fixed (A) and cryofixed (HPF-FS) (B) samples showing branches (arrows). <b>C</b>. Slice of an electron tomographic reconstruction of the stack that shows an Y-shape cisterna (arrow). <b>D</b>. Three-dimensional model of the Golgi region shown in C (the complete model is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185557#pone.0185557.g003" target="_blank">Fig 3C</a>). Apart from the Y-shape cisterna (arrow) there is a connection between two adjacent cisternae (arrowhead). <b>E</b>. Slice of a reconstruction of a stack made up of dilated cisternae, where a cisterna projects a branch passing through neighbouring cisternae (arrow). <b>F</b>. 3D model of the tomogram reconstruction shown in E (the complete model is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185557#pone.0185557.g003" target="_blank">Fig 3B</a>). Volumes of the calculated tomograms (x,y,z): A: 4095 x 4095 x 186 pixels, B: 4095 x 4095 x 400 pixels, pixel size 0.59 nm.