Microbial binding site on FH19-20.

<p><i>Pseudomonas aeruginosa</i> (<b>A</b>), <i>Haemophilus influenzae</i> (<b>B</b>), <i>Bordetella pertussis</i> (<b>C</b>), <i>Streptococcus pneumoniae</i> (<b>D</b>), and <i>Candida albicans</i> (<b>E</b>) were coated to microtitre plates and binding of ¹²⁵I-FH19-20 was measured in the presence of serial dilutions of 14 mutant proteins. Bound radioactivity was measured and the IC50 values (the 50% inhibitory concentration in µM) were determined by fitting these measurements to inhibition curves (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003308#ppat.1003308.s002" target="_blank">Figure S2</a>). Means of the reciprocal values of IC50 (1/IC50) with SDs of three individual experiments performed in triplicate are shown with difference compared to the wildtype (wt) calculated by a t-test. *p<0.05, **p<0.01, ***p<0.001.