Binding phenotypes of natural <i>S. enterica</i> subspecies I FimH variants.

<p>Static adhesion of <i>S.</i> Typhimurium LBH4 transformed with plasmids encoding different variants of FimH or plasmids carrying <i>fimH</i> deletion (fimHΔ) to Man-BSA (red bars) and anti-FimH^SE antibody (grey bars). The binding of ³H- labeled bacteria was determined as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002733#s4" target="_blank">Material and Methods</a>. Data are the means ± SD of triplicates from one representative experiment of three experiments that were performed. The strain tags of systemically invasive serovars are in red and the non-invasive ones in black. Bacterial binding was >95% inhibitable in the presence of 50 mM methyl-D-mannopyranoside (not shown). * The non-binding FimH variants of <i>S.</i> Gallinarum were not tested in this study.