CADM1 is responsible for mast cell adhesion to primary human lung fibroblasts.

<p>HMC-1 cells and HLMCs were transduced with SP4, SP1, CADM1 shRNA, GFP or luciferase control shRNA using adenoviral delivery, followed by the study of adhesion to HLFs. A) Downregulation of CADM1 reduced HMC-1 cell adhesion to HLFs (n = 3–6 for different groups), while overexpression of SP4 increased it. *p<0.05, **p<0.01 compared to CADM1 shRNA. #p<0.05 compared to SP4. B) Downregulation of CADM1 reduced adhesion of HLMCs (n = 4) to HLFs (n = 5), while SP4 overexpression increased adhesion. *p<0.05 compared to CADM1 shRNA. #p<0.05 compared to SP4. C) HMC-1 cells pre-incubated with anti-CADM1 9D2 Ab adhered less to HLFs compared to controls (n = 3). **p<0.01 compared to 9D2. D) and E) Scatter plots with regression model parameters for the CADM1 surface expression levels and the degree of adhesion to HLFs by HMC-1 cells (D) and HLMCs (E), which include the data for transduced cells from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061579#pone-0061579-g003" target="_blank">Fig. 3</a> including SP4, luciferase control shRNA and CADM1 shRNA transduction groups (20 points in D and 14 points in E) and their expression data published previously in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061579#pone-0061579-g002" target="_blank">Fig 2b</a> of reference 26 for HMC-1 cells and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061579#pone-0061579-g001" target="_blank">Fig. 1</a> in this paper for HLMCs.