Glycan receptor-binding properties of mWF10:T189A and mWF10:L226Q.

<p><b>A</b>, Dose-dependent direct glycan array binding of mWF10:T189A HA (<i>left</i>) shows increased specificity to the human receptor 6′SLN-LN when compared to WF10 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059550#pone-0059550-g001" target="_blank"><b>Figure 1A</b>, left panel</a>). Tissue-binding of mWF10:T189A HA (<i>right</i>) shows extensive staining of apical surface of human tracheal section and minimal staining of human alveolar section mWF10: T189A HA in <i>green</i> against propidium iodide in <i>red</i>). <b>B</b>, Dose-dependent direct glycan array binding of mWF10:L226Q HA (<i>left</i>) shows a complete reversal in binding from human (6′ SLN-LN) to avian receptors (3′SLN-LN and 3′SLN-LN-LN). Tissue staining (<i>right</i>) shows poor staining of apical surface of human tracheal epithelia and extensive staining of human alveolar sections by mWF10:L226Q HA (in green) shown against propidium iodide staining (in red).