MARCKS phosphorylation at the ED and S25 in E8 chick embryo retinas stimulated with PMA.

<p>(<b>A</b>) Sequential Western-blot immunodetections for MARCKS, pED-MARCKS and S25p-MARCKS of E8 chick embryo retinas treated with different concentrations of PMA for 30 minutes. There are two controls: untreated and 0,1% DMSO. (<b>B–C</b>) Single confocal sections of chick retina cryosections, immunolabeled for MARCKS, pED-MARCKS and S25p-MARCKS. Insets on the right show a higher magnification untreated retinas, at the vitreal margin to highlight the localization of pED-MARCKS and S25p- MARCKS in ganglion cells. Arrowheads, mitotic cells at the apical border of the differentiating retina. gcl, ganglion cell layer; inl, inner nuclear layer; ipl, inner plexiform layer; ofl, optic fiber layer. Scale bars: 20 µm.