Cdk and GSK3β inhibitors and MARCKS phosphorylation at S25 in cultured retina neuroblasts.

<p>(<b>A–D</b>) Bar diagrams representing the relative phosphorylation of MARCKS at S25, or GSK3β at S9, as percent of control, after treatment with Cdk and GSK3β inhibitors. Chick E8 retina cells were cultured for 7 hours and treated during the final hour or two with 100 µM olomoucine (A), 25 µM roscovitine (B), or 20 mM LiCl (C and D). Values in C and D were taken from the same experiments. Control cultures were treated with 0.05% DMSO (A and B) or 20 mM NaCl (C and D) for 1 or 2 hours. The values are represented as mean±SEM, *p≤0.05. Statistical significance was determined using the nonparametric tests Kruskal-Wallis and Wilcoxon-Man Whitney. Numbers inside the bars show the “n” for each case (independent experiments). Representative Western-blots are shown in A′–D′, sequential immunodetections are shown in different pseudo-colors. (<b>E</b>) Scheme showing the time-course of treatments.