<i>pvcA</i> mutation reduces the expression of <i>cupB</i> and <i>cupC</i> gene clusters.

<p>The relative level of expression for each gene in PW4830 (Δ<i>pvcA</i>) compared to MPAO1 was determined by RT-qPCR. The quantity of cDNA in different samples was normalized using 30S ribosomal RNA (<i>rplS</i>) as an internal standard. <b>A.. </b><i>pvcA</i> mutation in MPAO1 significantly reduces the expression of all genes of the <i>cupB</i> gene cluster. Order and direction of transcription of <i>cupB1-6</i> on the MPAO1 chromosome is indicated by block arrows above the graph. <b>B.. </b><i>pvcA</i> mutation in MPAO1 significantly reduces the expression of all <i>cupC</i> genes. Order and direction of transcription of <i>cupC1-3</i> on the MPAO1 chromosome is indicated by block arrows above the graph. Values in A and B represent the average of triplicate PCR experiments conducted on three independently obtained RNA preparations ± SEM (n = 3); <i>P</i><0.01 (**); <i>P</i><0.001 (***).