Significantly enriched pathways with progressive patterns identified using gene set enrichment analysis.

<p>A) Upregulated Pathways B) Downregulated Pathways. The solid dots indicate significantly affected pathways (False Discovery Rate <25%) identified from across group comparisons (N1 vs. N2, N1 vs. M and N2 vs. M) at a particular time point (T0, T1 and T2). The asterisks represent significance and directionality of enrichment (P value<0.09 *, P value<0.05 **, P value<0.01 ***) identified from within group comparisons at different time points (T0 vs. T1, T0 vs. T2, T1 vs. T2). The red and green color asterisks indicate up- and down-regulated enrichment of pathways respectively. The heatmaps depicting relative expression of selected genes from representative pathways are shown in panels on the right side. Gene expression is shown with a pseudo color scale (−3 to 3) with red and green colors denoting increased and decreased relative expression respectively. Pathways with progressive patterns were enriched (up- or down- regulated) in N2 and M groups with greater significance of enrichments in M group. Furthermore, increasing enrichment over time within one session of RR elicitation was observed in M group.