Evaluation of cytokine production by splenic cells from <i>T.cruzi</i> infected mice in response to TLR agonists.

<p>Cytokine levels in spleen cell culture stimulated or not with LPS (1 µg/ml), Pam3Cys (1 µg/ml) or CpG DNA (1 µg/ml) from either control (non-infected) or infected C57BL/6 WT, TLR2^−/−, and TLR9^−/− mice seven days post-infection. Supernatants from spleen cell cultures from C57BL/6 WT (A), TLR2^−/− (B) and TLR9^−/− mice (C) were analyzed for IL-12/IL-23p40 or TNF-α after 48h. Data are representative of two independent experiments. *p<0.05 indicates statistical significance when comparing cytokine release by spleen cells stimulated or not with TLR agonist in a same group (infected or not infected mice).