The effect of PGJ2 or Mdivi-1 treatment on cortical neurons under normal and OGD conditions.

<p>(A) Shows a representative Drp1 western blot of control, and OGD cells with and without PGJ₂ treatment. With the non-denaturing western blot method an additional band (above the 227 kDa molecular weight marker) appeared on the western blot due to PGJ₂ treatment both in control, and in OGD samples, but it was not detectable after 24 h reoxygenation. (B) Shows the changes in viability of the cells due to PGJ₂ treatment and 3 h OGD. PGJ₂ treatment decreased cell viability in 15 and 20 µmol/L concentrations and further decreased cell viability after 3 h OGD in 10, 15, and 20 µmol/L concentrations. (C) Shows a representative Drp1 western blot of control, and OGD cells with and without Mdivi-1 treatment. The treatment did not change Drp1 expression in our experiments. (D) Shows the changes in viability of the cells due to Mdivi-1 treatment and 3 h OGD. Mdivi-1 was not protective in 3 h OGD, and at higher doses (75, 100 µmol/L) it decreased cell viability in control neurons. Each panel represents <i>n</i>≥3 independent experiments. *p<0.05 vs. control, † p<0.05 vs. untreated OGD, Co = Control, Drp1 = Dynamin-related protein 1, Mdivi-1 = Mitochondrial division inhibitor-1, PGJ₂ = 15-deoxy-D12,14-prostaglandin J₂, R/O = reoxygenation, OGD = oxygen-glucose deprivation.