Maturation of DCs induced by particle-bound PADRE does not involve calcineurin.

<p>Day-5 DCs were collected and incubated with particle-bound PADRE, particle-bound P2C or ionomycin for additional 2 days in the presence of absence of cyclosporine A. On day 7 the cells were stained with antibodies for CD80, CD86, CD83 and HLA-DR, and analyzed by flow cytometry. The culture supernatants were analyzed for IL-12p70 by ELISA. The histograms in (<b>A</b>) show the expression of CD80, CD86, CD83 and HLA-DR of a representative donor, diagram (<b>B</b>) shows the secretion of IL-12p70. Independent experiments were done with cells from seven different healthy donors.