IRF4 co-localizes with EBNA3C in human cells.

<p>A) 0.3 million HEK-293 cells plated on coverslips and transiently transfected with Control vector, GFP-EBNA3C and Flag-IRF4 expression vectors by using Lipofectamine 2000 transfection reagent. B) BJAB, BJAB10, LCL1 cells were plated on slides and air-dried. C) 0.3 million HEK-293 cells were grown on coverslips and transiently transfected with different truncated domains of GFP-EBNA3C (residues 1–365, 366–620, 621–992) and Flag-IRF4 expression vectors by using Lipofectamine 2000 transfection reagent. Cells were fixed using 3% PFA. Ectopic and endogenous expressions of IRF4 was detected using anti-Flag (M2)-antibody (1∶200 dilution) and IRF4 specific antibody (1∶50 dilution) respectively, followed by anti-Rabbit Alexa Fluor 594 (red) as secondary antibody. Endogenous EBNA3C was detected using A10 ascites (1∶150 dilution) followed by anti-mouse Alexa Fluor 488 (green). DAPI (49, 69-diamidino-2-phenylindole) was used (1∶500 dilution) to stain nuclei. The images were captured by Olympus Fluoview confocal microscope.