EBNA3C co-localizes with RASSF1A in nuclear compartments in human cells.

(A) EBNA3C co-localized with RASSF1A in Saos-2 cells. 0.1 million Saos-2 cells were plated on coverslips and transfected with Flag-tagged EBNA3C, Myc-tagged RASSF1A or RASSF1A-ΔRA separately or together by using jetPRIME transfection reagent. 24h post-transfection, cells were subjected to immunofluorescence assays. (B) EBNA3C co-localized with endogenous RASSF1A in B-cells. BJAB, BJAB7, BJAB10, LCL1, and LCL2 cells were plated on the slide and air-dried. The cells were fixed and subjected to immunofluorescence assays as described in materials and methods.