Triclabendazole extends the yeast chronological life span.

<p>(A) Growth with triclabendazole or vehicle (DMSO, 0.1%). Drug was added during lag-phase (arrow). Values are the mean ± standard deviation (SD) of the three independent experiments. (B) Chronological aging assay (survival as a function of time). Drug was added during lag-phase, and then cells were pre-grown for 48 h ( = day 0 of life span assay) (2, 5 μM DMSO and triclabendazole, TCBZ) or for 72 h ( = day 0 of life span assay) (10 μM triclabendazole). Viability was measured as a function of time. Values are the mean ± SD of the four independent experiments. (C) Plot of life span <i>versus</i> phase when drug was added. Life span values (<i>t_1/2</i>) are the mean ± SD of the four independent experiments. *, <i>p</i><0.001 (versus DMSO).