a<p>PCR conditions: 94°C 5 min, 40×(94°C 1 min, 37–50°C 1 min, 72°C 1 min 50 s), 72°C 5 min;</p>b<p>PCR conditions: 94°C 5 min, 35×(94°C 1 min, 54–60°C 1 min, 72°C 1 min 50 s), 72°C 5 min;</p>c<p>PCR conditions: 94°C 5 min, 35×(94°C 45 s, 58°C 45 s, 72°C 45 s), 72°C 5 min;</p>d<p>PCR conditions: 94°C 5 min, 35×(94°C 1 min, 52–60°C 1 min, 72°C 1 min), 72°C 5 min;</p>e<p>PCR conditions: 94°C 5 min, 35×(94°C 45 s, 52°C 45 s, 72°C 45 s), 72°C 5 min.</p
<p>* Primers for β-actin were used for both non-quantitative and real-time PCR.</p><p><i>(A) Non-qua...
<p>All templates were initially denatured for 5 min at 94°C, and after completing all cycles, were e...
a<p>The PCR thermal cycle profile consisted of 1 cycle of denaturation for 5 min at 95°C; 35 cycles ...
<p>PCR conditions: initial 3 minute 95°C denaturation, followed by 37°C of 1 min at 95°C, 30 seconds...
<p>*PCR conditions: denature at 94°C for 3 min, 1 cycle; denature at 94°C for 1 min, anneal at 57°C ...
<p>Note: List of other primers used in Quantitative Real-time PCR is given in <a href="http://www.pl...
<p>PCR conditions: 40 cycles of 30 sec denaturation at 95°C, 30 sec annealing at (a) 55°C or (b) 60°...
(A) Trial primers for target DNA amplification (target C 4mer, 7mer, and 8mer listed in Table 3). (B...
(A) Trial primers for target DNA amplification (target C 4mer, 7mer, and 8mer listed in Table 3). (B...
*<p>Restriction sites (underlined) introduced in primers: <u>GGTACC</u> (<i>Kpn</i>I), <u>GGATCC</u>...
*<p>F: forward, R: reverse.</p>1<p>Primers from Folz <i>et al. Am. J. Respir. Cell Mol. Biol.</i> 20...
<p>Predicted fragment sizes (approximate, in bp) and PCR cycle profiles (temperature in °C: time in ...
Nucleotide sequences, amplification sites, GenBank accession numbers, amplimer sizes and PCR reactio...
<p><sup><i>1</i></sup>D: A, G or T; H: A, C or T: M: A or C; R: A or G; W: A or T; Y: C or T; V: A, ...
<p>List of primers that were used for qPCR assays and an end-point RT-PCR assay.</p
<p>* Primers for β-actin were used for both non-quantitative and real-time PCR.</p><p><i>(A) Non-qua...
<p>All templates were initially denatured for 5 min at 94°C, and after completing all cycles, were e...
a<p>The PCR thermal cycle profile consisted of 1 cycle of denaturation for 5 min at 95°C; 35 cycles ...
<p>PCR conditions: initial 3 minute 95°C denaturation, followed by 37°C of 1 min at 95°C, 30 seconds...
<p>*PCR conditions: denature at 94°C for 3 min, 1 cycle; denature at 94°C for 1 min, anneal at 57°C ...
<p>Note: List of other primers used in Quantitative Real-time PCR is given in <a href="http://www.pl...
<p>PCR conditions: 40 cycles of 30 sec denaturation at 95°C, 30 sec annealing at (a) 55°C or (b) 60°...
(A) Trial primers for target DNA amplification (target C 4mer, 7mer, and 8mer listed in Table 3). (B...
(A) Trial primers for target DNA amplification (target C 4mer, 7mer, and 8mer listed in Table 3). (B...
*<p>Restriction sites (underlined) introduced in primers: <u>GGTACC</u> (<i>Kpn</i>I), <u>GGATCC</u>...
*<p>F: forward, R: reverse.</p>1<p>Primers from Folz <i>et al. Am. J. Respir. Cell Mol. Biol.</i> 20...
<p>Predicted fragment sizes (approximate, in bp) and PCR cycle profiles (temperature in °C: time in ...
Nucleotide sequences, amplification sites, GenBank accession numbers, amplimer sizes and PCR reactio...
<p><sup><i>1</i></sup>D: A, G or T; H: A, C or T: M: A or C; R: A or G; W: A or T; Y: C or T; V: A, ...
<p>List of primers that were used for qPCR assays and an end-point RT-PCR assay.</p
<p>* Primers for β-actin were used for both non-quantitative and real-time PCR.</p><p><i>(A) Non-qua...
<p>All templates were initially denatured for 5 min at 94°C, and after completing all cycles, were e...
a<p>The PCR thermal cycle profile consisted of 1 cycle of denaturation for 5 min at 95°C; 35 cycles ...